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Membranes were blocked with Tris-buffered saline (TBS) and 0.1% Tween-20 (TBS/T) containing 5% bovine serum albumin (Sangon Biotech Co., Ltd., Shanghai, China) at 37C for 2 h, and incubated overnight at 4C with primary antibodies (Abcam, Cambridge, MA, USA

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Rhbdd3, a member of the rhomboid protease family, negatively regulates DC activation and maintains the balance of Treg and Th17 cells by inhibiting IL-6 production in DCs, thus contributing to the prevention of autoimmune diseases [97]

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Active secretion begins at the blastocyst stage, enabling hCG detection in the maternal circulation approximately 10 days after fertilisation, with the highest values reached between the 10th and 11th week of pregnancy before declining through the remainder of the first trimester